Identification of walnut (Juglans regia) leaf spot agent by its production of species-specific phytotoxin

Aim. For the purpose of correct species and internal identification isolated strains bacteria that cause walnut leaf spotting, molecular biological genetic detection their ability to synthesize phytotoxin syringomycin have been carried out. Methods. It has used microbiological (PCR) methods. Results. The presence (syrD) secretion genes was established in spot pathogen typical strain Pseudomonas syringae pv. UCM B-1027Т. Biotesting by these revealed similarity antagonistic action from B-1027T, which indicates lipodepsinopeptide – syringomycin. Conclusions. Based on results previous researches presented this work, were identified as a members syringae.